进行了一项开放 、随机、人体模型研究 ，以比较外用抗生素 、伤口保护剂和消毒剂对伤口愈合速度和细菌生长的影响，采用改良的方法 ，使用氢氧化铵诱导的接种金黄色葡萄球菌的皮内水泡。研究中的每位志愿者都有六个水泡伤口（每只前臂三个） ，每天两次涂抹三联抗生素（新霉素、多粘菌素 B 、杆菌肽）软膏或其他四种测试药物中的一种。对照伤口未经治疗。治疗后，所有伤口均用封闭敷料覆盖。评估每个伤口的愈合时间（100% 上皮化） 。两次治疗后对伤口进行细菌培养 。用三联抗生素软膏治疗的受污染水泡伤口愈合速度明显快于用任何消毒剂治疗的伤口和未接受治疗的伤口只有新霉素-多粘菌素B-杆菌肽组合在两次应用后（在被金黄色葡萄球菌污染后16至24小时内）才有效消除伤口的细菌污染 。用三联抗生素治疗的伤口的整体临床外观和愈合率优于研究中除其他抗生素软膏外的所有治疗（和无治疗） 。
Comparison of topical antibiotic ointments, a wound protectant, and antiseptics for the treatment of human blister wounds contaminated with Staphylococcus aureus
An open, randomized, human-model study was conducted to compare the effects of topical antibiotics, a wound protectant, and antiseptics on the rate of wound healing and bacterial growth using a modification of a method employing ammonium hydroxide-induced intradermal blisters inoculated with Staphylococcus aureus. Each volunteer in the study had six blister wounds (three per forearm) to which a triple antibiotic (neomycin, polymyxin B, bacitracin) ointment or one of four other test agents was applied twice a day. A control wound remained untreated. All wounds were covered with an occlusive dressing after treatment. The time to healing (100 percent epithelialization) was evalsuated for each wound. Wounds were cultured for bacterial growth after two treatments. Contaminated blister wounds treated with the triple antibiotic ointment healed significantly faster (mean nine days) than wounds treated with any antiseptic and those receiving no treatment. Only the neomycin-polymyxin B-bacitracin combination effectively eliminated bacterial contamination of the wounds after two applications (within 16 to 24 hours after contamination with Staphylococcus aureus). The overall clinical appearance and healing rates of wounds treated with the triple antibiotic were ranked superior to all treatments (and no treatment) except the other antibiotic ointment in the study.